BIOINFORMATICS


SHOTGUN METAGENOMICS AND MICROBIOME PROFILING
  • Shallow Shotgun Sequencing: Our shallow shotgun sequencing optimized for high-biomass samples with a low to moderate host DNA background offers a very cost effective solution for large scale microbiome projects for community surveillance.

  • Deep Shotgun Sequencing: Together with our high quality bias-free shotgun sequencing, and most extensive and curated EzBioCloud reference database, our deep shotgun microbiome service offers highly resolved and precise multi-kingdom taxonomic profiling, with comprehensive community characterization and direct functional gene and pathway annotation. Our database includes species with validly published names, candidates, potential new species (> 3000 new species) and uncultured phylotypes.

EzBiome Shallow and Deep shotgun microbiome service offers highly resolved Subspecies-level microbiome profiling with the most extensive taxonomic coverage. Our database includes species with validly published names, candidates, potential new species (> 3000 new species) and uncultured phylotypes. 

Therefore, together with our high quality bias-free shotgun sequencing, most extensive and curated EzBioCloud reference database, we offer highly resolved and precise multi-kingdom taxonomic profiling, with comprehensive community characterization and direct functional gene and pathway annotation.

AMPLICON (16S/ITS)

Amplicon Sequencing: Our 16S microbiome service offers low-bias amplicon sequencing of bacteria (V1-V3, V3-V4, V4) and fungi (ITS2) community with the most extensive taxonomic (>96% species-level classification rate) and functional (using >16,000 KEGG orthologs) coverage.

EzBiome 16S microbiome service offers you highly resolved species-level microbiome profiling with the most extensive taxonomic coverage. Our database includes species with validly published names, candidates, potential new species (> 3000 new species) and uncultured phylotypes. It offers great coverage on the human microbiome: >96% species-level identification can be achieved. You can also predict KEGG-based 16S functional profiles using >10,000 species genome making our predictions far more accurate and insightful than the competition.

Therefore, together with our high quality bias-free amplicon sequencing, most extensive and curated EzBioCloud reference database, we offer highly resolved and precise species-level taxonomic profiling, with comprehensive prediction of functional landscape to take your microbiome research to the next level.

WHOLE GENOME ANALYSIS & COMPARATIVE GENOMICS

Full-length genomic analysis service for strain isolates. Results provide genetic information and functional/evolutionary characteristics of the genes through comparison to related species.

EzBiome’s WG service is perfect for studying pathogenicity, antibiotics resistance, microbiome-related functions, diagnostic biomarker discovery, and secondary metabolism. Or you can take your analysis to the next level with comparative genomics, where you select several genomes and go beyond simple discovery. 

All the open reading frames (ORFs) and RNA and their detailed information annotated in the analysis pipeline are viewed through an easy to use genome browser. You’ll receive publication-ready genome maps and alignments at the click of a button. A Pairwise Ortholog Matrix is generated by a complete calculation of the pairwise ortholog detection within a data set. Two algorithms for ortholog detection methods, namely Reciprocal Best Hit (RBH) and ORF-independents, are used to generate the tables. A Venn diagram provides Pan-genome orthologous groups (POG) sharedness in a pan-genome and is useful for gaining insight into how pan-genome ortholog genes are present differentially across the genomes in a pan-genome. Conduct pathway enrichment analysis to uncover metabolic pathways that are only present in one group but not in another utilizing a KEGG database.

Highlights

  • Readily browse genomic content
  • Export pertinent statistics (contigs, CDS, GC ratio, rRNA, tRNA, etc.) as Excel or FASTA format
  • Create publication ready genome maps
  • Detect lateral gene transfer
  • Various Pan-genome Orthologous analysis
  • Statistical biomarker discovery
CUSTOM MICROBIAL & MICROBIOME BIOINFORMATICS

Since the advent of Next Generation Sequencing scientist have uncovered associations between a plethora of factors and microbial taxa. Sequence-based approaches to study the microbiome such as shotgun and amplicon seqeuncing have a drawback that the necessary bioinformatic analyses are complex. We understand that your research and experiments are important and unique, that’s why we offer custom bioinformatics Learn More.

Contact Our Experts

Do you want a quote or you have a question? Contact us because we’re always here to help